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At day 4 post infection, cell supernatant containing the released virus was harvested, centrifuged at 500 g for 5 min, filtered through a 0.22 M sterile vacuum filtration system, aliquoted and stored at 80 C until further use
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Assess the results: If the smoke odor persists after the initial treatment, you may need to repeat the process
One particular brand of e-cigarette, called the JUUL, has become very popular with teenagers and young adults
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